CKAP5

Protein-coding gene in the species Homo sapiens

CKAP5

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
4QMI, 4QMJ

Identifiers

Aliases

CKAP5, CHTOG, MSPS, TOG, TOGp, ch-TOG, cytoskeleton associated protein 5

External IDs

OMIM: 611142; MGI: 1923036; HomoloGene: 8844; GeneCards: CKAP5; OMA:CKAP5 - orthologs

Gene location (Human)

Chr.	Chromosome 11 (human)^[1]

Band	11p11.2	Start	46,743,048 bp^[1]
Band	11p11.2	End	46,846,308 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 2 (mouse)^[2]

Band	2\|2 E1	Start	91,357,107 bp^[2]
Band	2\|2 E1	End	91,451,009 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

ventricular zone

ganglionic eminence

oocyte

right testis

stromal cell of endometrium

Achilles tendon

left testis

secondary oocyte

prefrontal cortex

cerebellar hemisphere

Top expressed in

tail of embryo

genital tubercle

ventricular zone

gastrula

ganglionic eminence

cerebellar cortex

maxillary prominence

secondary oocyte

mandibular prominence

zygote

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protein binding cadherin binding microtubule plus-end binding
Cellular component	cytoplasm cytosol centrosome spindle pole membrane microtubule cytoskeleton gamma-tubulin complex microtubule organizing center microtubule plus-end cytoskeleton chromosome, centromeric region kinetochore chromosome spindle nucleolus plasma membrane protein-containing complex
Biological process	RNA transport establishment or maintenance of microtubule cytoskeleton polarity cell division spindle organization G2/M transition of mitotic cell cycle cell cycle sister chromatid cohesion centrosome cycle microtubule polymerization centrosome duplication ciliary basal body-plasma membrane docking regulation of G2/M transition of mitotic cell cycle microtubule depolymerization
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


9793


75786

Ensembl


ENSG00000175216


ENSMUSG00000040549

UniProt


Q14008


A2AGT5

RefSeq (mRNA)


NM_014756 NM_001008938


NM_001165989 NM_029437

RefSeq (protein)


NP_001008938 NP_055571


NP_001159461 NP_083713

Location (UCSC)

Chr 11: 46.74 – 46.85 Mb

Chr 2: 91.36 – 91.45 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene.^[5]^[6]^[7] It is the homolog of the Xenopus protein XMAP215^[8] and is also known as ch-Tog.

It has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK (KIF2C), and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity.^[9]

Interactions

CKAP5 has been shown to interact with TACC1.^[10]^[11]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000175216 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000040549 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H (Jul 1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.
^ Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors". Eur. J. Biochem. 234 (2): 406–13. doi:10.1111/j.1432-1033.1995.406_b.x. PMID 8536682.
^ "Entrez Gene: CKAP5 cytoskeleton associated protein 5".
^ Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC 379257. PMID 14718566.
^ Barr AR, Gergely F (2008). "MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics". Mol. Cell. Biol. 28 (23): 7199–211. doi:10.1128/MCB.01040-08. PMC 2593372. PMID 18809577.
^ Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (Nov 2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID 14603251. S2CID 10536264.
^ Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (Apr 2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC 1222467. PMID 11903063.

External links

Human CKAP5 genome location and CKAP5 gene details page in the UCSC Genome Browser.

Basto R, Gergely F, Draviam VM, Ohkura H, Liley K, Raff JW (2007). "Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans". J. Cell Sci. 120 (Pt 7): 1278–87. doi:10.1242/jcs.000604. PMID 17376965.
Kosturko LD, Maggipinto MJ, D'Sa C, Carson JH, Barbarese E (2005). "The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2". Mol. Biol. Cell. 16 (4): 1938–47. doi:10.1091/mbc.E04-08-0709. PMC 1073673. PMID 15703215.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC 379257. PMID 14718566.
Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID 14603251. S2CID 10536264.
Gergely F, Draviam VM, Raff JW (2003). "The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells". Genes Dev. 17 (3): 336–41. doi:10.1101/gad.245603. PMC 195983. PMID 12569123.
Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC 1222467. PMID 11903063.
Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLOS ONE. 7 (12): e51442. Bibcode:2012PLoSO...751442N. doi:10.1371/journal.pone.0051442. PMC 3520847. PMID 23251535.
Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID 23666085. S2CID 3025200.