DNA2L

Protein-coding gene in the species Homo sapiens
DNA2
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

5EAY

Identifiers
AliasesDNA2, DNA2L, hDNA replication helicase/nuclease 2
External IDsOMIM: 601810; MGI: 2443732; HomoloGene: 6124; GeneCards: DNA2; OMA:DNA2 - orthologs
Gene location (Human)
Chromosome 10 (human)
Chr.Chromosome 10 (human)[1]
Chromosome 10 (human)
Genomic location for DNA2
Genomic location for DNA2
Band10q21.3Start68,414,064 bp[1]
End68,472,121 bp[1]
Gene location (Mouse)
Chromosome 10 (mouse)
Chr.Chromosome 10 (mouse)[2]
Chromosome 10 (mouse)
Genomic location for DNA2
Genomic location for DNA2
Band10|10 B4Start62,782,805 bp[2]
End62,809,964 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • secondary oocyte

  • gonad

  • testicle

  • ventricular zone

  • ganglionic eminence

  • right lobe of liver

  • Epithelium of choroid plexus

  • rectum

  • trabecular bone

  • stromal cell of endometrium
Top expressed in
  • fetal liver hematopoietic progenitor cell

  • primitive streak

  • abdominal wall

  • otic placode

  • epiblast

  • otic vesicle

  • tibiofemoral joint

  • migratory enteric neural crest cell

  • mandibular prominence

  • medial ganglionic eminence
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • DNA binding
  • 4 iron, 4 sulfur cluster binding
  • nucleotide binding
  • helicase activity
  • iron-sulfur cluster binding
  • metal ion binding
  • 5'-flap endonuclease activity
  • DNA helicase activity
  • ATPase activity
  • protein binding
  • catalytic activity
  • 5'-3' DNA helicase activity
  • nuclease activity
  • endonuclease activity
  • site-specific endodeoxyribonuclease activity, specific for altered base
  • hydrolase activity
  • ATP binding
  • single-stranded DNA helicase activity
  • RNA binding
Cellular component
  • nucleoplasm
  • gamma DNA polymerase complex
  • mitochondrial nucleoid
  • nucleus
  • mitochondrion
  • cytoplasm
Biological process
  • mitotic telomere maintenance via semi-conservative replication
  • mitochondrial DNA repair
  • nucleic acid phosphodiester bond hydrolysis
  • DNA double-strand break processing
  • mitochondrial DNA replication
  • cellular response to DNA damage stimulus
  • DNA replication checkpoint signaling
  • positive regulation of DNA replication
  • metabolism
  • base-excision repair
  • DNA replication, removal of RNA primer
  • DNA replication
  • telomere maintenance
  • DNA duplex unwinding
  • DNA repair
  • G-quadruplex DNA unwinding
  • t-circle formation
  • telomere maintenance via semi-conservative replication
  • DNA replication, Okazaki fragment processing
  • replication fork reversal
  • regulation of signal transduction by p53 class mediator
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

1763

327762

Ensembl

ENSG00000138346

ENSMUSG00000036875

UniProt

P51530

Q6ZQJ5

RefSeq (mRNA)

NM_001080449

NM_177372

RefSeq (protein)

NP_001073918

NP_796346

Location (UCSC)Chr 10: 68.41 – 68.47 MbChr 10: 62.78 – 62.81 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

DNA2-like helicase is an enzyme that in humans is encoded by the DNA2 gene.[5][6][7] Dna2, a homolog of DNA2KL present in budding yeast, possesses both helicase and nuclease activity, with which it helps catalyze early steps in homologous recombination.[8]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000138346 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036875 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Eki T, Okumura K, Shiratori A, Abe M, Nogami M, Taguchi H, Shibata T, Murakami Y, Hanaoka F (Mar 1997). "Assignment of the closest human homologue (DNA2L:KIAA0083) of the yeast Dna2 helicase gene to chromosome band 10q21.3-q22.1". Genomics. 37 (3): 408–10. doi:10.1006/geno.1996.0581. PMID 8938459.
  6. ^ Masuda-Sasa T, Polaczek P, Campbell JL (Dec 2006). "Single strand annealing and ATP-independent strand exchange activities of yeast and human DNA2: possible role in Okazaki fragment maturation". J Biol Chem. 281 (50): 38555–64. doi:10.1074/jbc.M604925200. PMID 17032657.
  7. ^ "Entrez Gene: DNA2L DNA2 DNA replication helicase 2-like (yeast)".
  8. ^ Mimitou, EP; Symington, LS (May 2009). "Nucleases and helicases take center stage in homologous recombination". Trends in Biochemical Sciences. 34 (5): 264–272. doi:10.1016/j.tibs.2009.01.010. PMID 19375328.

Further reading

  • Masuda-Sasa T, Imamura O, Campbell JL (2006). "Biochemical analysis of human Dna2". Nucleic Acids Res. 34 (6): 1865–75. doi:10.1093/nar/gkl070. PMC 1428797. PMID 16595800.
  • Kim JH, Kim HD, Ryu GH, et al. (2006). "Isolation of human Dna2 endonuclease and characterization of its enzymatic properties". Nucleic Acids Res. 34 (6): 1854–64. doi:10.1093/nar/gkl102. PMC 1428795. PMID 16595799.
  • Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
  • Deloukas P, Earthrowl ME, Grafham DV, et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature. 429 (6990): 375–81. Bibcode:2004Natur.429..375D. doi:10.1038/nature02462. PMID 15164054.
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
  • Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.


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