Hydroxydechloroatrazine ethylaminohydrolase

In enzymology, a hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3) is an enzyme that catalyzes the chemical reaction

4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H₂O ${\displaystyle \rightleftharpoons }$ N-isopropylammelide + ethylamine

Thus, the two substrates of this enzyme are 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine and H₂O, whereas its two products are N-isopropylammelide and ethylamine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in compounds that have not been otherwise categorized within EC number 3.5. The systematic name of this enzyme class is 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase. Other names in common use include AtzB, and hydroxyatrazine ethylaminohydrolase. This enzyme participates in atrazine degradation.

• Boundy-Mills KL, de Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ (March 1997). "The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway". Applied and Environmental Microbiology. 63 (3): 916–23. PMC 168384. PMID 9055410.