PCBP2

Protein-coding gene in the species Homo sapiens

PCBP2

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
2AXY, 2JZX, 2P2R, 2PQU, 2PY9

Identifiers

Aliases

PCBP2, HNRNPE2, HNRPE2, hnRNP-E2, poly(rC) binding protein 2

External IDs

OMIM: 601210; MGI: 108202; HomoloGene: 74536; GeneCards: PCBP2; OMA:PCBP2 - orthologs

Gene location (Human)

Chr.	Chromosome 12 (human)^[1]

Band	12q13.13	Start	53,452,102 bp^[1]
Band	12q13.13	End	53,481,162 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 15 (mouse)^[2]

Band	15 F3\|15 57.61 cM	Start	102,470,539 bp^[2]
Band	15 F3\|15 57.61 cM	End	102,500,061 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

ganglionic eminence

ventricular zone

stromal cell of endometrium

left testis

right testis

left ovary

right adrenal cortex

left lobe of thyroid gland

right lobe of thyroid gland

right ovary

Top expressed in

neural layer of retina

epiblast

hand

ventricular zone

somite

molar

thymus

abdominal wall

tail of embryo

endocardial cushion

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	DNA binding enzyme binding nucleic acid binding ubiquitin protein ligase binding protein binding RNA binding C-rich single-stranded DNA binding
Cellular component	extracellular exosome membrane focal adhesion nucleoplasm cytoplasm nucleus cytosol postsynaptic density
Biological process	mRNA splicing, via spliceosome negative regulation of defense response to virus proteasome-mediated ubiquitin-dependent protein catabolic process defense response to virus mRNA metabolic process negative regulation of type I interferon production immune system process innate immune response viral RNA genome replication IRES-dependent viral translational initiation RNA metabolic process
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


5094


18521

Ensembl


ENSG00000197111


ENSMUSG00000056851

UniProt


Q15366


Q61990

RefSeq (mRNA)

NM_031989 NM_001098620 NM_001128911 NM_001128912 NM_001128913
NM_001128914 NM_005016


NM_001103165 NM_001103166 NM_001174073 NM_011042

RefSeq (protein)

NP_001092090 NP_001122383 NP_001122384 NP_001122385 NP_001122386
NP_005007 NP_114366


NP_001096635 NP_001096636 NP_001167544 NP_035172 NP_001361700

Location (UCSC)

Chr 12: 53.45 – 53.48 Mb

Chr 15: 102.47 – 102.5 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Poly(rC)-binding protein 2 is a protein that in humans is encoded by the PCBP2 gene.^[5]

Function

The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.

It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene.^[6]

In humans, the PCBP2 gene overlaps with TUC338, a transcribed ultra-conserved element implicated in Hepatocellular carcinoma.^[7]

Interactions

PCBP2 has been shown to interact with HNRPK,^[8] PTBP1, and HNRNPL.^[8]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000197111 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000056851 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tommerup N, Leffers H (May 1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A". Genomics. 32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID 8833161.
^ "Entrez Gene: PCBP2 poly(rC) binding protein 2". Archived from the original on 2024-04-30. Retrieved 2017-09-07.
^ Braconi C, Valeri N, Kogure T, Gasparini P, Huang N, Nuovo GJ, Terracciano L, Croce CM, Patel T (2011-01-11). "Expression and functional role of a transcribed noncoding RNA with an ultraconserved element in hepatocellular carcinoma". Proceedings of the National Academy of Sciences of the United States of America. 108 (2): 786–91. Bibcode:2011PNAS..108..786B. doi:10.1073/pnas.1011098108. PMC 3021052. PMID 21187392.
^ ^a ^b Kim JH, Hahm B, Kim YK, Choi M, Jang SK (May 2000). "Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm". J. Mol. Biol. 298 (3): 395–405. doi:10.1006/jmbi.2000.3687. PMID 10772858.

Kiledjian M, Wang X, Liebhaber SA (1995). "Identification of two KH domain proteins in the alpha-globin mRNP stability complex". EMBO J. 14 (17): 4357–64. doi:10.1002/j.1460-2075.1995.tb00110.x. PMC 394520. PMID 7556077.
Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains". Eur. J. Biochem. 230 (2): 447–53. doi:10.1111/j.1432-1033.1995.tb20581.x. PMID 7607214.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Blyn LB, Swiderek KM, Richards O, Stahl DC, Semler BL, Ehrenfeld E (1996). "Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 11115–20. Bibcode:1996PNAS...9311115B. doi:10.1073/pnas.93.20.11115. PMC 38293. PMID 8855318.
Funke B, Zuleger B, Benavente R, Schuster T, Goller M, Stévenin J, Horak I (1996). "The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins". Nucleic Acids Res. 24 (19): 3821–8. doi:10.1093/nar/24.19.3821. PMC 146158. PMID 8871564.
Blyn LB, Towner JS, Semler BL, Ehrenfeld E (1997). "Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA". J. Virol. 71 (8): 6243–6. doi:10.1128/JVI.71.8.6243-6246.1997. PMC 191892. PMID 9223526.
Kiledjian M, DeMaria CT, Brewer G, Novick K (1997). "Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex". Mol. Cell. Biol. 17 (8): 4870–6. doi:10.1128/MCB.17.8.4870. PMC 232339. PMID 9234743.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Czyzyk-Krzeska MF, Bendixen AC (1999). "Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA". Blood. 93 (6): 2111–20. doi:10.1182/blood.V93.6.2111.406k24_2111_2120. PMID 10068686.
Wang Z, Day N, Trifillis P, Kiledjian M (1999). "An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro". Mol. Cell. Biol. 19 (7): 4552–60. doi:10.1128/MCB.19.7.4552. PMC 84253. PMID 10373504.
Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog". J. Biol. Chem. 274 (35): 24849–57. doi:10.1074/jbc.274.35.24849. PMID 10455157.
Kim JH, Hahm B, Kim YK, Choi M, Jang SK (2000). "Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm". J. Mol. Biol. 298 (3): 395–405. doi:10.1006/jmbi.2000.3687. PMID 10772858.
Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins". Genomics. 67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID 10936052.
Chang CJ, Luh HW, Wang SH, Lin HJ, Lee SC, Hu ST (2002). "The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein". DNA Cell Biol. 20 (9): 569–77. doi:10.1089/104454901317094981. PMID 11747608. Archived from the original on 2023-09-28. Retrieved 2022-10-14.
Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA". J. Biol. Chem. 277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID 12011088.
Walter BL, Parsley TB, Ehrenfeld E, Semler BL (2002). "Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication". J. Virol. 76 (23): 12008–22. doi:10.1128/JVI.76.23.12008-12022.2002. PMC 136902. PMID 12414943.
Abraham RS, Geyer SM, Price-Troska TL, Allmer C, Kyle RA, Gertz MA, Fonseca R (2003). "Immunoglobulin light chain variable (V) region genes influence clinical presentation and outcome in light chain-associated amyloidosis (AL)". Blood. 101 (10): 3801–8. doi:10.1182/blood-2002-09-2707. PMID 12515719. S2CID 38371635.
Pickering BM, Mitchell SA, Evans JR, Willis AE (2003). "Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo". Nucleic Acids Res. 31 (2): 639–46. doi:10.1093/nar/gkg146. PMC 140511. PMID 12527772.

PDB gallery

1ztg: human alpha polyC binding protein KH1
2axy: Crystal Structure of KH1 domain of human Poly(C)-binding protein-2 with C-rich strand of human telomeric DNA