Thymidylate 5'-phosphatase

The enzyme thymidylate 5′-phosphatase (EC 3.1.3.35) catalyzes the reaction

thymidylate + H₂O ${\displaystyle \rightleftharpoons }$ thymidine + phosphate

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name is thymidylate 5′-phosphohydrolase. Other names in common use include thymidylate 5′-nucleotidase, deoxythymidylate 5′-nucleotidase, thymidylate nucleotidase, deoxythymidylic 5′-nucleotidase, deoxythymidylate phosphohydrolase, and dTMPase.

• Aposhian HV, Tremblay GY (1966). "Deoxythymidylate 5′-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C". J. Biol. Chem. 241 (21): 5095–101. PMID 4958986.