PPM1A

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Proteinska fosFataza, Mg²⁺/Mn²⁺ zavisna, 1A

PDB prikaz baziran na 1a6q.

Dostupne strukture

1A6Q, 3FXJ, 3FXK, 3FXL, 3FXM, 3FXO

Identifikatori

Simboli

PPM1A; PP2C-ALPHA; PP2CA; PP2Calpha

Vanjski ID

OMIM: 606108 MGI: 99878 HomoloGene: 56428 GeneCards: PPM1A Gene

EC broj

3.1.3.16

Ontologija gena
Molekularna funkcija	• vezivanje jona magnezijuma • aktivnost proteinske serin/treoninske fosfataze • aktivnost prenosnika signala • proteinsko vezivanje • vezivanje proteinskog C-terminusa • hidrolazna aktivnost • vezivanje jona mangana • aktivnost kalmodulin-zavisne proteinske fosfataze • R-SMAD vezivanje
Celularna komponenta	• membranska frakcija • nukleus • citozol • naponom kontrolisani kompleks kalcijumskog kanala • kompleks proteinske serin/treoninske fosfataze • neuronska projekcija
Biološki proces	• blokada ćelijskog ciklusa • prenos signala • signalni put insulinskog receptora • negativna regulacija formiranja kompleksa SMAD proteina • signalni put Wnt receptora • defosforilacija • pozitivna regulacija signalnog puta Wnt receptora

Pregled RNK izražavanja

podaci

Ortolozi

Vrsta

Čovek

Miš

Entrez

5494

19042

Ensembl

ENSG00000100614

ENSMUSG00000021096

UniProt

P35813

P49443

RefSeq (mRNA)

NM_021003.4

NM_008910.3

RefSeq (protein)

NP_066283.1

NP_032936.1

Lokacija (UCSC)

Chr 14:
60.71 - 60.77 Mb

Chr 12:
73.86 - 73.9 Mb

PubMed pretraga

[1]

[2]

PPM1A (Proteinska fosfataza 1A) je enzim koji je kod ljudi kodiran PPM1A genom.^[1]^[2]

Protein kodiran ovim genom je član PP2C familije Ser/Thr proteinskih fosfataza. Članovi PP2C familije su poznati kao negativni regulatori ćelijskog puta kojim se odgovara na stres. Ova fosfataza defosforiliše, i negativno reguliše aktivnosti MAP kinaza i MAP kinaza kinaza. Pokazano je da inhibira aktivaciju p38 i JNK kaskade kinaza indukovanu stresovima životne sredine. Ova fosfataza može takođe da defosforiliše ciklin-zavisne kinaze, i stoga može da učestvuje u kontroli ćelijskog ciklusa. Prekomerno izražavanje ove fosfataze može da aktivira izražavanje tumor supresornih gena TP53/p53, što dovodi do G2/M blokade ćelijkog ciklusa i apoptoze. Poznate su tri alternativno splajsovane transkriptne varijante koje kodiraju dve distinktne izoforme.^[2]

Interakcije

PPM1A formira interakcije sa metabotropnim glutamatnim receptor 3.^[3] PPM1A može da prekine TGF-beta signalizaciju putem inaktivacije Smad3 defosforilacijom. Smad3 je esencijalna komponenta TGF-beta signalnog puta.

Reference

↑ Mann DJ, Campbell DG, McGowan CH, Cohen PT (Apr 1992). „Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences”. Biochim Biophys Acta 1130 (1): 100–4. PMID 1311954.
↑ ^2,0 ^2,1 „Entrez Gene: PPM1A protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform”.
↑ Flajolet, Marc; Rakhilin Sergey, Wang Hong, Starkova Natalia, Nuangchamnong Nina, Nairn Angus C, Greengard Paul (December 2003). „Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3”. Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (26): 16006–11. DOI:10.1073/pnas.2136600100. ISSN 0027-8424. PMC 307683. PMID 14663150.

Literatura

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Takekawa M, Maeda T, Saito H (1998). „Protein phosphatase 2Calpha inhibits the human stress-responsive p38 and JNK MAPK pathways.”. EMBO J. 17 (16): 4744–52. DOI:10.1093/emboj/17.16.4744. PMC 1170803. PMID 9707433.
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Ota T, Suzuki Y, Nishikawa T, et al. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs.”. Nat. Genet. 36 (1): 40–5. DOI:10.1038/ng1285. PMID 14702039.
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