PSMF1

Protein found in humans

PSMF1

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
2VT8, 4OUH

Identifiers

Aliases

PSMF1, PI31, proteasome inhibitor subunit 1

External IDs

OMIM: 617858; MGI: 1346072; HomoloGene: 38231; GeneCards: PSMF1; OMA:PSMF1 - orthologs

Gene location (Human)

Chr.	Chromosome 20 (human)^[1]

Band	20p13	Start	1,113,240 bp^[1]
Band	20p13	End	1,189,415 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 2 (mouse)^[2]

Band	2\|2 G3	Start	151,557,732 bp^[2]
Band	2\|2 G3	End	151,586,106 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

sperm

left testis

right testis

muscle of thigh

blood

gastrocnemius muscle

popliteal artery

tibial arteries

monocyte

islet of Langerhans

Top expressed in

seminiferous tubule

spermatid

secondary oocyte

zygote

primary oocyte

blood

sciatic nerve

interventricular septum

fetal liver hematopoietic progenitor cell

spermatocyte

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	proteasome binding protein binding endopeptidase inhibitor activity protein homodimerization activity protein heterodimerization activity
Cellular component	cytoplasm cytosol membrane nucleoplasm endoplasmic reticulum proteasome complex proteasome core complex perinuclear region of cytoplasm
Biological process	regulation of cellular amino acid metabolic process antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent ubiquitin-dependent protein catabolic process regulation of mRNA stability positive regulation of canonical Wnt signaling pathway protein polyubiquitination stimulatory C-type lectin receptor signaling pathway tumor necrosis factor-mediated signaling pathway MAPK cascade Fc-epsilon receptor signaling pathway NIK/NF-kappaB signaling negative regulation of proteasomal protein catabolic process anaphase-promoting complex-dependent catabolic process T cell receptor signaling pathway negative regulation of canonical Wnt signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process Wnt signaling pathway, planar cell polarity pathway negative regulation of endopeptidase activity negative regulation of G2/M transition of mitotic cell cycle protein deubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process transmembrane transport regulation of transcription from RNA polymerase II promoter in response to hypoxia post-translational protein modification regulation of hematopoietic stem cell differentiation interleukin-1-mediated signaling pathway regulation of mitotic cell cycle phase transition
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


9491


228769

Ensembl


ENSG00000125818


ENSMUSG00000032869

UniProt


Q92530 Q5QPM9


Q8BHL8

RefSeq (mRNA)

NM_006814 NM_178578 NM_178579 NM_001323407 NM_001323408
NM_001323409 NM_001323410


NM_144889 NM_212446 NM_001305244

RefSeq (protein)

NP_001310336 NP_001310337 NP_001310338 NP_001310339 NP_006805
NP_848693


NP_001292173 NP_997611

Location (UCSC)

Chr 20: 1.11 – 1.19 Mb

Chr 2: 151.56 – 151.59 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Proteasome inhibitor PI31 subunit is a protein that in humans is encoded by the PSMF1 gene.^[5]^[6]

Function

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMF1 encodes a protein termed PI31 that binds to both 20S and 26S proteasomes. PI31 was originally identified by its ability to inhibit the hydrolysis of small synthetic peptides in a cell free assay, but it stimulates 26S proteasome activity both in vitro and in vivo.^[7]^[8] PI31 can promote the assembly of 26S proteasomes from 19S and 20S sub-particles, and it also serves as an adapter to couple proteasomes with motor proteins to mediate fast axonal transport in neurons.^[9] Inactivation of PMSF1 in yeast, plants, fruit flies and mice attenuates protein degradation and stimulates accumulation of ubiquitinated protein aggregates. Furthermore, loss of PSMF1 function in mice causes progressive neuronal dysfunction, synaptic degeneration and eventually neuronal cell death.^[10] Alternative transcript variants have been identified for this gene.^[6]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000125818 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000032869 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tanahashi N, Kawahara H, Murakami Y, Tanaka K (Apr 1999). "The proteasome-dependent proteolytic system". Molecular Biology Reports. 26 (1–2): 3–9. doi:10.1023/A:1006909522731. PMID 10363639. S2CID 38305744.
^ ^a ^b "Entrez Gene: PSMF1 proteasome (prosome, macropain) inhibitor subunit 1 (PI31)".
^ Chu-Ping, M. "Purification and characterization of a protein inhibitor of the 20S proteasome (macropain)". BBA. 1119: 303–11. doi:10.1016/0167-4838(92)90218-3. PMID 1312359.
^ Bader, Maya (2011). "A conserved F box regulatory complex controls proteasome activity in Drosophila". Cell. 145. doi:10.1016/j.cell.2011.03.021. PMC 3108249. PMID 21529711.
^ Liu, Kai (2019). "PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development". Dev Cell. 50: 509–524. doi:10.1016/j.devcel.2019.06.009. PMC 6702053. PMID 31327739.
^ Minis, Adi (2019). "The proteasome regulator PI31 is required for protein homeostasis, synapse maintenance, and neuronal survival in mice". Proc Natl Acad Sci U S A. 116: 24639–24650. doi:10.1073/pnas.1911921116. PMC 6900516. PMID 31754024.

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